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Agilent technologies xcelligence real-time cell analysis (rtca) system
Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the <t>xCELLigence</t> <t>RTCA</t> and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays
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Agilent technologies xcelligence real-time cell analysis (rtca
Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the <t>xCELLigence</t> <t>RTCA</t> and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays
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Agilent technologies xcelligence real- time cell analysis sp reader
Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the <t>xCELLigence</t> <t>RTCA</t> and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays
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Agilent technologies xcelligence real‐time cell analysis (rtca) dp (dual purpose) system
Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the <t>xCELLigence</t> <t>RTCA</t> and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays
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Agilent technologies xcelligence real-time cell analysis system
Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the <t>xCELLigence</t> <t>RTCA</t> and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays
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Roche xcelligence real time cell analysis rtca
Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the <t>xCELLigence</t> <t>RTCA</t> and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays
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Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the xCELLigence RTCA and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays

Journal: Virology Journal

Article Title: Evaluation of three alternative methods to the plaque reduction neutralizing assay for measuring neutralizing antibodies to dengue virus serotype 2

doi: 10.1186/s12985-024-02459-y

Figure Lengend Snippet: Comparison of DENV-2 neutralization titers obtained by the MTT-based microneutralization, the xCELLigence RTCA and the iPA-FRNT assays with the standard PRNT assay. Linear regression and scatterplot of neutralizing titers comparing the evaluated assay with standard PRNT at neutralization endpoints of 50% (left panel), 70% (middle panel) and 90% (right panel). Panel A-C : MTT-based microneutralization assay. Non-linearity was observed for two of three neutralization endpoints (NT 70 and NT 90 ). ( D-F ) xCELLigence RTCA assay. ( G-I ) iPA-FRNT assay. At all three neutralization endpoints, positive correlation was observed for both the xCELLigence RTCA and iPA-FRNT assays

Article Snippet: The xCELLigence real-time cell analysis (RTCA) system (Agilent Technologies) utilizes electrical impedance to continuously monitor changes in cell proliferation, morphology, adhesion, and viability caused by virus-induced cytopathic effects (CPE) and has been used to measure nAbs against West Nile virus, H1N1, and human enterovirus 71 [ , , ].

Techniques: Comparison, Neutralization, Plaque Reduction Neutralization Test, Microneutralization Assay

Comparison of alternative methods to the plaque reduction neutralizing assay for measuring neutralizing antibodies to DENV-2

Journal: Virology Journal

Article Title: Evaluation of three alternative methods to the plaque reduction neutralizing assay for measuring neutralizing antibodies to dengue virus serotype 2

doi: 10.1186/s12985-024-02459-y

Figure Lengend Snippet: Comparison of alternative methods to the plaque reduction neutralizing assay for measuring neutralizing antibodies to DENV-2

Article Snippet: The xCELLigence real-time cell analysis (RTCA) system (Agilent Technologies) utilizes electrical impedance to continuously monitor changes in cell proliferation, morphology, adhesion, and viability caused by virus-induced cytopathic effects (CPE) and has been used to measure nAbs against West Nile virus, H1N1, and human enterovirus 71 [ , , ].

Techniques: Comparison, Neutralizing Assay, Virus, Cell Culture